A study completed in 2009 using .01% TB500 w/w eye drops demonstrated increased speed of healing following eye surgery in diabetics. This population was specifically investigated due to well known healing complications in diabetics, and their predilection for eye issues, specifically diabetic retinopathy for eye issues, specifically diabetic retinopathy.. The study showed no serious side ef The study showed no serious side effects in any patient, although headache, fects in any patient, although headache, dizziness and insomnia occurred more in the study group than the control. Of specific interest, human diabetic retinopathic corneas express substantially less endogenous TB500 than a normal cornea, suggesting an inherent deficiency in ability for diabetics to heal following eye injury or surgery diabetics to heal following eye injury or surgery.. [5] [5] Similarly Similarly, chronic dry-eye patients were treated with , chronic dry-eye patients were treated with TB500 eye drops, and all TB500 eye drops, and all in the study had improvement in symptoms, with rare and minimal complications. [6]

TB500 was studied in the setting of chronic hepatitis B combined with nonalcoholic fatty liver disease (NAFLD). While TB500 had no correlation with HepB V no correlation with HepB Virus levels nor liver function tests (AST irus levels nor liver function tests (AST, , ALALTT, , TG), there was a negative correlation with inflammation and TG), there was a negative correlation with inflammation and fibrosis scores – meaning that the lower the level of TB500, the worse the inflammation and fibrosis. This provided preliminary data that TB500 may be beneficial in the setting of certain liver diseases. [7]

A human voluntary safety and tolerance study was performed in 2010, and showed that intravenous (IV) doses from 42mg up to 1260mg daily for 14 days had no treatment related adverse effects, and no evidence of dose related toxicity. [8]

 Rhinovirus (a common cold virus) was introduced to healthy volunteers, and blood tests were taken during a five-day period. This showed that serum cortisol rose along with thymosin alpha 1 and TB500 on the fifth day following intentional exposure. Simultaneously, t-lymphocytes (CD3+), cytotoxic/suppressor (CD8+), and natural killer (CD16+) cells all rose. This suggests a cooperative effect among systemic and cellular immune response related to thymosin and respiratory virus exposure. [9]

Kidney disease often includes inflammation, fibrosis, and complications from diabetes. TB500 has been studied in mice with and without kidney disease; in healthy mice, levels of TB500 did not affect healthy kidney tissue, but in those with known kidney disease, low levels of disease, low levels of TB500 correlated with worsening disease. TB500 correlated with worsening disease. That is, low levels of natural That is, low levels of natural TB500 accelerated kidney disease. TB500 accelerated kidney disease. The study suggested that endogenous TB500 levels function to protect the kidney and slow disease progression. [10]

 TB500 has been studied specifically in healing of skin and wounds, showing acceleration in healing of skin in burns, diabetic ulcers, elderly subjects, pressure ulcers, stasis ulcers, and epidermolysis wounds, in both animal and human subjects. The study shows improvement in angiogenesis (blood vessel formation), anti-inflammatory activity, and to increase platelet aggregation at wound sites. [11] A meta-analysis (study of studies) conducted in 2015 showed broad applicability of TB500 in various disease processes, including improvement of tissue regeneration, repair of the heart after heart attack, healing of the brain following stroke, trauma and neurological diseases. Further, it showed clinical improvement in kidney and liver diseases, and repair of spinal cord, bone and ligament injuries, as well as reducing consequences of aging and viral infection. [12] TB500 treated mice were found to have substantially improved strength in healed fractures compared to untreated mice. Treated mice showed a 41% increase in peak force to failure, and healed fractures were 25% stiffer than untreated mice. 21-day postfracture imaging showed improvement up to 26% better than untreated subjects. They also showed 23% smaller callus, and a 31% increase in trabecular bone area/total callus area. Overall, TB500 demonstrated substantially faster healing, with betterhealed injury sites when compared to placebo subjects. [13]

 Spinal cord injury has been studied in rats, with potential applicability to human subjects. In a 2014 study, TB500 was administered to rats 30 minutes, 3 days, or 5 days after mild compression induced spinal cord injury in rats. Locomotor and behavioral assessments were all markedly improved with the TB500 treated group. Inflammatory cytokines were reduced, the size of scars were markedly reduced, and levels of myelin proteins were 57.8% above the control group. The study suggested that, with the known safety of TB500, that it should be considered for treatment of spinal cord injury in humans. [14]

While no animal model perfectly mimics humans for head injury research, rats are considered a close second due to our understanding of their behaviors. TB500 has been studied in traumatic brain injury (TBI), and has been demonstrated to markedly improve function after improve function after TBI. TBI. TB500 exerts both neuroprotective and neurorestorative ef TB500 exerts both neuroprotective and neurorestorative effects on rats, including proliferation of blood fects on rats, including proliferation of blood flow, new brain cells, and new connections between brain cells. [15]

Multiple sclerosis has also been shown, in rat models, to benefit from administration of TB500. Zhang et al, in their 2016 study,, showed that administration of TB500 significantly increased the number of newly generated oligodendrocytes and reduced axonal damage in the mice compared with control group. Importantly, the newly generated oligodendrocytes remyelinated axons, and this correlated with functional improvement. [16

TB-500 is a synthetic peptide version of the naturally occuring healing protein present in about all human and mammalian cells known as Thymosin Beta 4 (TB4). Research has shown TB 500 to have beneficial effects on wound healing, injury recovery, flexibility, and inflammation. These desirable effects are accomplished through TB 500’s influence on blood cell and blood vessel development, cellular differentiation, and cell migration. A major part of its healing capability stems from the peptide’s regulation of actin, a cell building protein essential to healing and wound repair.

TB-500 Dosage

TB500 comes as a freeze dried (lyophilized) powder. Research has shown that best results are achieved when bacteriostatic sodium chloride 0.9% is used to reconstitute thymosin peptides. TB 500 dosages can range from 5mg to 20 mg per week. Administration is generally divided into 2-3 injections per week. Once the peptide is built up in the system, the weekly dosage is generally lowered a bit to a maintenance dosage of about half or less of the original weekly dose. Administration of maintenance dosage can be made once per week.

TB500 is typically injected subcutaneously, though it can also be injected into muscle if desired.

How to Store TB 500: Store the product at room temperature in a non-humid environment. The vial should be shielded from light. An opened vial can be kept for up to 8 days refrigerated.

What is TB-500

TB 500 research illustrates multiple properties that have provided the impetus for a worldwide series of clinical trials of potential effectiveness for thymosin beta-4 in promoting repair of wounds in skin, cornea and heart. Thymosin beta 4 is produced in the thymus gland and is found in high concentrations in wound fluids. As such it has been observed to facilitate healing of injured tissue in the body.

TB-500 is a naturally occurring peptide found in high concentrations in blood platelets, wound fluid and other tissues. TB-500 peptides for research are not growth factors; rather major actin regulating protein peptides. TB 500 has been found to play an important role in protection, regeneration and remodeling of injured and/or damaged tissues. The gene for Thymosin Beta-4 has also been found to be one of the first to be stimulated after an incident of injury.

Present in almost all human and animal cells, Thymosin Beta 4 is naturally occurring wound healing peptide. TB 500 is a synthetically made version of TB4 that facilitates healing and recovery by helping build new blood vessels, muscle tissue fibers, blood cells, and facilitating cell migration. For the overworked or injured individual, TB 500 offers extremely enticing wound healing effects.

How Does TB 500 Work?

TB 500 is able to help recovery by exerting effects on actin. By up-regulating this cellular protein, TB 500 is able to capitalize on actin’s positive effects. These include the promotion of cell migration and proliferation. As a result, new blood vessels form and inflammation can be regulated so that healing and recovery are optimised. In addition, TB 500’s unique molecular structure allows it to travel long distances in the body. This enables it to exert a “systemic” effect, seeking out and helping those areas of the body suffering injury. Further beneficial results of TB 500 administration can include reduced inflammation and enhanced flexibility. There have even been some reports of regrowing and darkening of hair.

What TB 500 Side Effects Are There?

There are many positive effects associated with Tb500 use, including enhanced recovery, reduced inflammation, and improved flexibility. The peptide works very effectively to aid recovery of slow healing injuries such as those to connective tissue, i.e. tendons and ligaments. Muscle injuries and injuries to skin are greatly helped as well. From reports from users and researchers around the globe, TB 500 seems to cause very few, if any, serious negative side effects.

Most peptides generally can produce a lightheadedness or nauseous feeling upon injection. Also, flu-like symptoms are prevalent among many other peptides as well. However, the incidence of these negative side effects occurring with TB500 administration is reported to be very low, if at all. TB 500 appears to be a very well tolerated peptide among users.

The information presented on this web site is not intended to take the place of your personal physician’s advice and is not intended to diagnose, treat, cure or prevent any disease.